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p irs 1 tyr1222  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p irs 1 tyr1222
    P Irs 1 Tyr1222, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+irs+1+tyr1222/Phospho-IRS-1+(Tyr1222)+Antibody/pm41886457-271-17-20
    Average 93 stars, based on 22 article reviews
    p irs 1 tyr1222 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Gut glucose assimilation governs glucose homeostasis through reciprocal interaction between glucose and amino acid transporter.
    Article Snippet: Equal amounts of protein (20 μg per lane) were separated by SDS–PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, Millipore). .. After blocking, the membranes were incubated with the following primary antibodies: p-IRS-1 (Ser307) (2381s, Cell Signaling Technology), p-IRS-1 (Tyr1222) (3066s, Cell Signaling Technology), IRS-1 (2382s, Cell Signaling Technology), p-PI3K (17366s, Cell Signaling Technology), PI3K (ab278545, Abcam), p-Akt (4060s, Cell Signaling Technology), Akt (4691s, Cell Signaling Technology), β-actin (60009-1-Ig, Proteintech), GAPDH (60004-1-Ig, Proteintech), GLUT1 (ab115730, Abcam), GLUT2 (PA5-77459, Invitrogen), GLUT3 (ab191071, Abcam), GLUT4 (ab188317, Abcam), GLUT5 (PA5-80024, Invitrogen), SGLT1 (PA5-37937, Invitrogen) and SLC6A19 (ab180516, Abcam). .. After washing and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology), the immune complexes were visualized using a chemiluminescence reagent.

    Incubation:

    Article Title: Gut glucose assimilation governs glucose homeostasis through reciprocal interaction between glucose and amino acid transporter.
    Article Snippet: Equal amounts of protein (20 μg per lane) were separated by SDS–PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, Millipore). .. After blocking, the membranes were incubated with the following primary antibodies: p-IRS-1 (Ser307) (2381s, Cell Signaling Technology), p-IRS-1 (Tyr1222) (3066s, Cell Signaling Technology), IRS-1 (2382s, Cell Signaling Technology), p-PI3K (17366s, Cell Signaling Technology), PI3K (ab278545, Abcam), p-Akt (4060s, Cell Signaling Technology), Akt (4691s, Cell Signaling Technology), β-actin (60009-1-Ig, Proteintech), GAPDH (60004-1-Ig, Proteintech), GLUT1 (ab115730, Abcam), GLUT2 (PA5-77459, Invitrogen), GLUT3 (ab191071, Abcam), GLUT4 (ab188317, Abcam), GLUT5 (PA5-80024, Invitrogen), SGLT1 (PA5-37937, Invitrogen) and SLC6A19 (ab180516, Abcam). .. After washing and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology), the immune complexes were visualized using a chemiluminescence reagent.



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